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KMID : 0380020130280060408
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 6 p.408 ~ p.414
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The Effect of Antioxidant and Antimicrobial Activity on the Extracted Its Material and Aloe vera L. Callus Culture by the Natural Fruit Juice
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Lee In-Soon
Bae Dong-Nyeok
Kwon Oh-Min
Han Gu-Tai
Kim Dae-Hwan
Oh Myeong-Won
Lee Ji-Hong
Moon Hae-yeon
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Abstract
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In order to obtain functional materials from aloe callus, we cultured Aloe vera L. leaf on MS medium added 0.2 mg/L IAA, 0.3 mg/L kinetin and 100 mg/L grape or/and apple juice for 30 days. While a callus differentiation during callus culture did not show, the cultured leaves were uniquely released extracellular material into the agar plate. After cultivation for 18 days, the cultured leaf and agar were harvested for extraction a functional material. The materials extracted were measured on the amount of total phenols, flavonoids and polysaccharides and determined on the antioxidant and antimicrobial activity. In result, callus extracts of additive free (CT) and added apple juice (2T) had more amount of phenol compound (659 μg/mL, 533 μg/mL) and flavonoid (580 μg/mL, 501 μg/mL) than natural leaf (p: 525 μg/mL, f: 301 μg/mL). However, the extract of natural leaf had the better effect of lipid peroxidation and polysaccharide content than the culture extract. All samples extracted had same effect on the nitrite scavenging activity. On the other hand, only 2T extract showed excellent 72% nitric oxide scavenging activity. The agar extract was also confirmed to contain polyphenol compound and polysaccharide content that had antioxidant and antimicrobial activity partly.
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KEYWORD
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Aloe vera L., Antioxidant activity, Antimicrobial activity, Polysaccharide, Polyphenol
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